Antibody Libraries available for screening

Naïve human antibody library (McCafferty Library - University of Cambridge)

The facility houses a high quality and very well characterised human naïve library of more than 1011 human antibodies licensed from the University of Cambridge. (Generated by John McCafferty and his colleagues at the Sanger Centre, Cambridge). This is one of the world's most important naïve human libraries, and has already been used to select, screen and sequence around 38,000 recombinant antibodies against almost 300 antigens, both prokaryotic and mammalian in origin, among which 7,200 are unique clones. Some of these clones are now progressing towards clinical studies.

This antibody library serves as an ideal screening platform for generating highly specific monoclonal antibodies with remarkable target recognition properties against defined antigenic conformations and epitopes.

SBF Human Semisynthetic Libraries

The SBF human semisynthetic libraries are available for royalty free, free for service selections. Two libraries are available for human antibody generation projects which have been validated using > 20 targets by the developers, generating positive hits with good sequence diversity.

The SBF semisynthetic libraries 1.0 and 2.0 sizes are ~7x1010

Semisynthetic libraries 1.0 - Germline DP47 VH FR1-FR3 + Naive VL (Vκ/Vλ)                                                

VH-CDR3 length variable – TRIM and biased codon approaches

Semisynthetic libraries 2.0 - DP47 VH TRI CDR1+CDR2 + Naive VL (Vκ/Vλ)

VH-CDR3 length variable - TRIM and biased codon approaches

Bespoke Immunised libraries

We specialise in the construction of bespoke immunised libraries from sources including the B cells of human donors, sheep, chicken, camel and traditional sources such as mouse and rabbit. The final choice of species may be influenced by the wishes of the client/researcher or depend on the specific requirements of an individual project.

These libraries are subjected to 'smart selection strategies' to enrich high affinity/specificity monoclonal phage antibodies, which then undergo antibody reformatting based on downstream applications. We have generated many immunised libraries (each approx. 1 x 109+ clones) and successfully employed them for selecting therapeutic, diagnostic and imaging antibodies for a range of projects involving academic and commercial partners.