Dr Ann Rajnicek

Dr Ann Rajnicek
Dr Ann Rajnicek

Dr Ann Rajnicek

BS, PhD, FRSB

Senior Lecturer

About

Qualifications

  • BSc Biology 
    1984 - Marygrove College (Detroit, MI USA) 
  • PhD Developmental Biology 
    1990 - Purdue University (W Lafayette, IN USA) 
    Thesis title: Effects of electric fields on wound healing and directed neurite growth

Memberships and Affiliations

Internal Memberships
  • Chair: School of Medicine, Medical Sciences and Nutrition, Staff Student Liaison Committee, Level 2 courses (2008-present)
  • Senior Personal Tutor: School of Medicine ,Medical Sciences and Nutrition (2021-present)
  • Co-lead, Co-founder: The IDEALL Group for Equality and Diversity (2016-present)
  • Member: Institute of Medical Sciences Equality, Diversity and Inclusion Committee (2016-present)
  • Member: University Senate (elected 2012-2016; re-elected 2016-2020)
  • Member: Self Assessment Team, Institute of Medical Sciences Athena Swan Application (2015-2019)
External Memberships

Senior Journal Editor/Media Editor: Bioelectricity (2019-present)(https://home.liebertpub.com/publications/bioelectricity/647)

External examiner: Glasgow University, Molecular and Cellular Biology/Biotechnology/Bioengineering undergraduate degrees (2017-2021)

Fellow: Royal Society of Biology (FRSB) 

Member: Society for Neuroscience

Member: British Society for Cell Biology

Member: Scottish Developmental Biology Group

Member: American Society for Cell Biology

Vice Chair: Gordon Research Conference on Bioelectrochemistry (2006)

Chair: Gordon Research Conference on Bioelectrochemistry (2008)

Board Member: Bioelectromagnetics Society (2008-2011)

Council Member: Bioelectrochemical Society (2009-2013)

 

Prizes and Awards

Yasuda Award for Excellence in Biomedical Research (Jan 2000) Society for Physical Regulation in Biology and Medicine.

Research

Research Overview

I am interested in the process by which cells use environmental conditions as guidance cues during development, wound healing and regeneration. My work has potential applications in tissue engineering and for devising clinical strategies to aid wound healing and nervous system repair. 

Guiding cells by DC electric fields

Cells exist within a naturally-occuring electric field, which results from the normal ion transport properties of polarized epithelia.  My research addresses the question of how cells use the electric field as a cue to direct cell migration and orientation.

Guiding cells by small substratum contours

The physical shape of the extracellular environment is usually not considered in the context of directional cell migration.  However, cells migrate along parallel substratum features on the scale of tens to hundreds of nanometers, which mimic the size of naturally ocurring features (such as individual collagen fibrils or oriented neuronal fibers).  I am interested in the process by which individual cells sense very small substratum features and how they translate the cues subsequently into directed migration.

Establishing a guidance heirarchy

Since electric fields and variation in substratum shape co-exist in vivo another interest is to determine the hierarchy of directional cues and the mechanisms that allow the cues to be selected/integrated by individual cells.  

Rho GTPases and growth cone guidance by an electric field

The Rho GTPases Rac, Rho and Cdc42 regulate cytoskeletal dynamics spatially and therefore control cell shape and the direction of cell migration.  We have shown that Rho GTPase-mediated cytoskeletal dynamics are essential for growth cone guidance by an electric field (Rajnicek et al., 2006 Journal of Cell Science 119:1723-1735;  Rajnicek et al. 2006 Journal of Cell Science 119: 1736-45). 

 

Current Research

 Growth cone guidance by substratum grooves

Growth cones are exquititely sensitive to substratum features on the scale of tens of nanometers deep but not all types of growth cones respond in the same way to identical features.  For example, embryonic rat hippocampal axons align at a right angle to a series of parallel grooves 130 nm deep and 1 um across but Xenopus spinal neuron growth cones migrate parallel to the same grooves.  I  am currently exploring the intracellular mechanisms growth cones use to detect small substratum features, including roles for Rho GTPases and the transcription factor Pax-6 (collaboration with Martin Collinson and Derryck Shewan, School of Medical Sciences, University of Aberdeen).


Guidance of epithelial cells by substratum nanotopograpy and electric fields is controlled by a rho/cdc42 switch 

Cells migrating to re-epithelialise a wound in the cornea migrate over non-planar surfaces within the context of a wound-induced DC electric field.  We have shown that corneal epithelial cells migrate parallel to nano-scale substratum grooves and that on planar quartz they migrate toward the cathode of a DC electric field.  By simultaneously challenging corneal cells with co-presented substratum grooves and an electric field oriented orthogonally we determined that the electric field was a more potent directional cue and that a cdc42/rho switch controls electrical/contact guidance priority.  This is relevant to the design of future therapies to aid wound healing as well as the basic mechanism for how cells sort/select/integrate simultaneous directonal guidance cues present in the normal extracellular environment.

Collaborations

Aberdeen- Prof Nieves Casan-Pastor, Dr W Huang, Dr D Shewan, Dr M Collinson, Dr H Wilson, Prof M Delibegovic 

Funding and Grants

IMS Spinal Research Fund -PhD Studentship "Electrical Control of Nerve Cell Growth"

School of Medical Sciences -PhD Studentship "The transcription factor Pax6 and neuronal guidance" (with M Collinson and D Shewan)

European Commission Specific Targeted Research or Innovation Project - "Development of a Bioelectrochemical device for CNS repair" (NERBIOS)  ~£220,000 (with C.D. McCaig)

BBSRC - Genetic control of epithelial cell migration and wound healing physiology (with M Collinson, C McCaig and M.Zhao) ~£643,000.

Teaching

Teaching Responsibilities

Course Coordinator:

AN4301- Developmental Neuroscience with Anatomy

PY4302- Developmental Neuroscience

BM3804- Neuroscience Research Topics

Non-course Teaching Responsibilities

Senior Personal Tutor for School of Medicine, Medical Sciences and Nutrition (undergraduates)

Personal Tutor and related pastoral care for undergraduates

Industrial Placement Tutor

Chair Level 2 undergraduate Staff Student Liaison Committee

Publications

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  • Electrical dimensions in cell science

    McCaig, C. D., Song, B., Rajnicek, A. M.
    Journal of Cell Science, vol. 122, no. 23, pp. 4267-4276
    Contributions to Journals: Articles
  • Chronic wound state exacerbated by oxidative stress in Pax6+/-aniridia-related keratopathy

    Ou, J., Walczysko, P., Kucerova, R., Rajnicek, A. M., McCaig, C. D., Zhao, M., Collinson, J. M.
    The Journal of pathology, vol. 215, no. 4, pp. 421-430
    Contributions to Journals: Articles
  • Alignment of corneal and lens epithelial cells by co-operative effects of substratum topography and DC electric fields

    Rajnicek, A. M., Foubister, L. E., McCaig, C. D.
    Biomaterials, vol. 29, no. 13, pp. 2082-2095
    Contributions to Journals: Articles
  • Prioritising guidance cues: Directional migration induced by substratum contours and electrical gradients is controlled by a rho/cdc42 switch

    Rajnicek, A. M., Foubister, L. E., McCaig, C. D.
    Developmental Biology, vol. 312, no. 1, pp. 448-460
    Contributions to Journals: Articles
  • Hardwiring the Brain: Endocannabinoids Shape Neuronal Connectivity

    Berghuis, P., Rajnicek, A. M., Morozov, Y. M., Ross, R. A., Mulder, J., Urban, G. M., Morony, K., Marsicano, G., Matteoli, M., Canty, A., Irving, A. J., Katona, I., Yanagawa, Y., Rakic, P., Lutz, B., Mackie, K., Harkany, T.
    Science, vol. 316, no. 5828, pp. 1212-1216
    Contributions to Journals: Articles
  • Growth cone steering by a physiological electric field requires dynamic microtubules, microfilaments and Rac-mediated filopodial asymmetry

    Rajnicek, A. M., Foubister, L. E., McCaig, C. D.
    Journal of Cell Science, vol. 119, pp. 1736-1745
    Contributions to Journals: Articles
  • Temporally and spatially coordinated roles for Rho, Rac, Cdc42 and their effectors in growth cone guidance by a physiological electric field.

    Rajnicek, A. M., Foubister, L. E., McCaig, C. D.
    Journal of Cell Science, vol. 119, pp. 1723-1735
    Contributions to Journals: Articles
  • Controlling cell behaviour electrically: current views and future potential

    McCaig, C. D., Rajnicek, A. M., Song, B., Zhao, M.
    Physiological Reviews, vol. 85, no. 3, pp. 943-978
    Contributions to Journals: Articles
  • Directing cell shape and migration by topographic and electrical signals

    Rajnicek, A. M.
    European Cells and Materials, vol. 4, no. SUPPL. 2, pp. 30-31
    Contributions to Journals: Articles
  • Has electrical growth cone guidance found its potential?

    McCaig, C. D., Rajnicek, A. M., Song, B., Zhao, M.
    Trends in Neurosciences, vol. 25, no. 7, pp. 354-359
    Contributions to Journals: Articles

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