CGEBM operates a dedicated single-cell genomics facility with stringently separated pre‐ and post‐PCR laboratories. We use a versatile range of approaches, including the Chromium 10X platform for sample preparation and the Countess II FL automated cell counter for accurate counting of viable and fluorescently labelled cells. We have expanded our capabilities to include SPLIT‐seq methods, specifically Parse Evercode, for scalable single-cell transcriptomics, even in fixed cells and FFPE samples.
These systems enable profiling up to millions of single cells or nuclei in a heterogeneous sample of interest from any organism, such as human, animal, plant or microbe, and from any tissue such as stem cells, blood, tumours, brain, organoid etc. Within CGEBM, these tools have successfully identified novel cell sub-populations and key marker genes from the transcriptomes of cell populations driving phenotypes of interest.
The Chromium 10X system can be used for single cell RNA-seq (gene expression), immune profiling, and ATAC-seq (global chromatin accessibility).
See the video below for details of one recent single cell genomics study.