Short-read sequencing

CGEBM operates Illumina MiSeq and NextSeq instruments for short read sequencing. Our facility also has Ion Torrent capabilities for short read applications.

The Illumina MiSeq provides sequencing capacity of up to 15 Gb output sequence with up to 25M reads and 300 bp paired end reads. This is an ideal platform for small genome sequencing and assembly, with paired ends improving de novo assemblies, as well as targeted and amplicon bi-directional sequencing requiring longer read lengths such as gene marker profiling of microbial communities (e.g. 16S, ITS) or custom amplicons. Multiplex up to 384 samples per run.

The Illumina NextSeq 500 provides sequencing capacity of up to 120 Gb output sequence with up to 400M reads and 150 bp paired end reads, for transcriptome, targeted resequencing, genotyping, and other applications.

The Illumina platforms use sequencing by synthesis (SBS) chemistries.

Our BioMek 4000 robotics systems allows high throughput sample and library preparation for large studies.