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The Rowett Institute

Dr Andreas Kolb

Dr Andreas Kolb
Dr Andreas Kolb
Dr Andreas Kolb

Senior Research Fellow

Accepting PhDs

About
Email Address
a.kolb@abdn.ac.uk
Telephone Number
+44 (0)1224 438645
School/Department
School of Medicine, Medical Sciences and Nutrition

Biography

2015-present: Senior Research Fellow, Rowett Institute, University of Aberdeen, Scotland, UK.

2006-2015: Research Fellow, Rowett Institute, University of Aberdeen, Scotland, UK.

2002-2005: Principal Investigator, Molecular Recognition Group, Hannah Research Institute, Ayr, Scotland, UK.

1997-2002: Investigator, Cell Physiology Group, Hannah Research Institute, Ayr, Scotland, UK.              

1992-1997: Postdoc, Institute of Virology and Immunology, University of Würzburg, Germany.

1989-1992: PhD Student, Institute of Molecular Animal Breeding, University of Munich, Germany.

Memberships and Affiliations

Internal Memberships

Postgraduate Research Coordinator, Rowett Institute

Chair, PGR Liaison Committee, School of Medicine Medical Sciences and Nutrition

External Memberships

Associate Fellow of The Higher Education Academy

Research

Research Overview

My research interest covers two overlapping research areas, namely:

  • Impact of secondary plant metabolites and toxins on metabolic health regulation, mediated by the gut microbiome and bile acid
  • Impact of nutrition in early life stages on adult metabolic health and aging.

Research Areas

Accepting PhDs

I am currently accepting PhDs in Nutrition and Health.

Please get in touch if you would like to discuss your research ideas further.

Email Me

Nutrition and Health

Supervising
Accepting PhDs
View Research Area

Current Research

Dietary and environmental impacts on metabolic health

The gut represents the main interface with which mammals interact with their environment. Bile acids are essential for the emulsification and uptake of dietary lipids and lipid-soluble vitamins. However, bile acids also act as signaling molecules via several nuclear receptors and G-protein coupled receptors. Many of these signaling events take place in the gut, but bile acids also circulate in serum and can also signal in other organs. Bile acids are synthesized in the liver, secreted via the gall bladder, and chemically modified by the gut microbiome to give rise to more than 20 different bile acids. We have found that dietary components can substantially alter the composition of bile acids in the gut and in serum. Environmental toxins (including mycotoxins and synthetic plastic compounds) can interact with bile acid signaling (directly or via the modulation of the microbiome) and derail the metabolic equilibrium. We are investigating the molecular mechanisms which are critical for bile acid signaling and how dietary components and toxins can influence these events. We apply this knowledge to develop cell-based assay systems to measure toxin concentrations in environmental and food samples.

 

Early life nutrition and metabolic health outcomes

Nutrition during early phases of life can have profound impacts on metabolic health in adulthood. Intrauterine growth restriction has been shown to alter body composition and epigenetic processes. This leads to increased susceptibility to obesity, type II diabetes and cardiovascular disease and shortened lifespan. Attenuated growth after birth, in contrast, is protective against aspects of the metabolic syndrome and extends lifespan. We are using in vitro and in vivo models of post-natal attenuated growth to study the molecular events which underlie the improved metabolic health outcomes and delayed aging. We apply this knowledge to develop pharmacological interventions to improve metabolic health.

Supervision

My current supervision areas are: Nutrition and Health.

Current PhD students:

Khulod Hasaballah

Amane Alaroud

Amanda Mathieson

Brendan Kesler

 

Past PhD students:

Alina Zitskaja (2016-2021)

Patrikas Pultinevicius (2015-2020)

Jennifer Harbottle (2013-2017)

Christopher Knowles (2012-2016)

Teaching

Teaching Responsibilities

ME2511: Student Selected Components Year 1, course coordinator

SM2501: Research Skills for Medical Sciences

SM2001: Foundation Skills for Medical Sciences

GN3502: Genetics

Non-course Teaching Responsibilities

Supervision of Honours and MSc projects

Publications

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  • Site specific insertion of a transgene into the murine α-casein (CSN1S1) gene results in the predictable expression of a recombinant protein in milk

    Knowles, C., Petrie, L., Warren, C., Lillico, S. G., Carlisle, A., Whitelaw, C. B. A., Kolb, A. F.
    Biotechnology Journal, vol. 19, no. 2, e2300287
    Contributions to Journals: Articles

  • The human genome at 20: how biology’s most-hyped breakthrough led to anticlimax and arrests

    MacKenzie, A., Kolb, A.
    Contributions to Specialist Publications

  • A cell-based assay system for activators of the environmental cell stress response

    Harbottle, J. A., Petrie, L., Ruhe, M., Houssen, W. E., Jaspars, M., Kolb, A. F.
    Analytical Biochemistry, vol. 592, 113583
    Contributions to Journals: Articles

  • Antiobesity functional leads and targets for drug development

    Anyanwu, G. O., Kolb, A. F., Bermano, G.
    Phytochemicals as Lead Compounds for New Drug Discovery. Egbuna, C., Kumar, S., Ifemeje, J., Ezzat, S., Kaliyaperumal, S. (eds.). Elsevier, pp. 143-160, 18 pages
    Chapters in Books, Reports and Conference Proceedings: Chapters

  • Folate deficiency promotes differentiation of vascular smooth muscle cells without affecting the methylation status of regulated genes

    Kolb, A. F., Petrie, L., Mayer, C. D., Pirie, L., Duthie, S. J.
    Biochemical Journal, vol. 476, no. 19, pp. 2769-2795
    Contributions to Journals: Articles

  • Recombinase-Mediated Cassette Exchange Using Adenoviral Vectors

    Kolb, A. F., Knowles, C., Pultinevicius, P., Harbottle, J. A., Petrie, L., Robinson, C., Sorrell, D. A.
    Methods in Molecular Biology, vol. 1642, pp. 127-150
    Contributions to Journals: Articles

  • Using the CRISPR/Cas9 system to understand neuropeptide biology and regulation

    Hay, E. A., Knowles, C., Kolb, A., MacKenzie, A.
    Neuropeptides, vol. 64, pp. 19-25
    Contributions to Journals: Articles

  • CD13/aminopeptidase N is a negative regulator of mast cell activation

    Zotz, J. S., Wölbing, F., Lassnig, C., Kauffmann, M., Schulte, U., Kolb, A. F., Whitelaw, B., Müller, M., Biedermann, T., Huber, M.
    The FASEB Journal, vol. 30, no. 6, pp. 2225-2235
    Contributions to Journals: Articles

  • Genetics and biosynthesis of milk proteins

    Vilotte, J. L., Chanat, E., Le Provost, F., Whitelaw, C. B., Kolb, A., Shennan, D. B.
    Advanced Dairy Chemistry: Volume 1A: Proteins: Basic Aspects, 4th Edition. McSweeny, P. L. H., Fox, P. F. (eds.). Springer US, pp. 431-461, 31 pages
    Chapters in Books, Reports and Conference Proceedings: Chapters

  • Folate deficiency enhances the inflammatory response of macrophages

    Kolb, A. F., Petrie, L.
    Molecular Immunology, vol. 54, no. 2, pp. 164-172
    Contributions to Journals: Articles

  • Behaviour of postnatally growth-impaired mice during malnutrition and after partial weight recovery

    Huber, R. C., Kolb, A. F., Lillico, S., Carlisle, A., Sandøe, P., Sørensen, D. B., Remuge, L., Whitelaw, B. C. A., Olsson, A. I. S.
    Nutritional Neuroscience, vol. 16, no. 3, pp. 125-134
    Contributions to Journals: Articles

  • Mammary gland development is delayed in mice deficient for aminopeptidase N

    Kolb, A. F., Sorrell, D., Lassnig, C., Lillico, S., Carlisle, A., Neil, C., Robinson, C., Müller, M., Whitelaw, C. B. A.
    Transgenic Research, vol. 22, no. 2, pp. 425-434
    Contributions to Journals: Articles

  • Milk lacking α-casein leads to permanent reduction in body size in mice

    Kolb, A. F., Huber, R. C., Lillico, S. G., Carlisle, A., Robinson, C. J., Neil, C., Petrie, L., Sorensen, D. B., Olsson, I. A. S., Whitelaw, C. B. A.
    PloS ONE, vol. 6, no. 7, e21775
    Contributions to Journals: Articles

  • Zinc finger recombinases with adaptable DNA sequence specificity

    Proudfoot, C., McPherson, A. L., Kolb, A. F., Stark, W. M.
    PloS ONE, vol. 6, no. 4, e19537
    Contributions to Journals: Articles

  • TRANSFER VEHICLE FOR PRODUCING PROTEINS IN TRANSGENIC ANIMALS

    Kolb, A. F.
    Patents: Patents

  • Recombinase mediated cassette exchange into genomic targets using an adenovirus vector

    Sorrell, D. A., Robinson, C. J., Smith, J., Kolb, A. F.
    Nucleic Acids Research, vol. 38, no. 11, e123
    Contributions to Journals: Articles

  • Hyperglycaemia: effects on expression of genes affecting epithelial differentiation and vascularisation

    Ghosh, S., Kolb, A., Collier, A., Simpson, C., Malik, I.
    Scottish Medical Journal, vol. 54, no. 4, pp. 48
    Contributions to Journals: Abstracts

  • Analysis of mammary specific gene locus regulation in differentiated cells derived by somatic cell fusion

    Robinson, C., Kolb, A.
    Experimental Cell Research, vol. 315, no. 3, pp. 508-522
    Contributions to Journals: Articles

  • Hyperglycaemia: Effect on Expression of Genes Affecting Epithelial Differentiation and Vascularisation

    Ghosh, S., Kolb, A., Collier, A., Simpson, C., Malik, I.
    Scottish Medical Journal, vol. 54, no. 1, pp. 52
    Contributions to Journals: Abstracts

  • EMMPRIN (basigin/CD147) Expression is not Correlated With MMP Activity During Adult Mouse Mammary Gland Development

    Szymanowska, M., Hendry, K. A. K., Robinson, C., Kolb, A.
    Journal of Cellular Biochemistry, vol. 106, no. 1, pp. 52-62
    Contributions to Journals: Articles

  • Prolactin inhibits cell loss and decreases matrix metalloproteinase expression in the involuting mouse mammary gland but fails to prevent cell loss in the mammary glands of mice expressing IGFBP-5 as a mammary transgene

    Flint, D. J., Boutinaud, M., Whitelaw, C. B. A., Allan, G. J., Kolb, A.
    Journal of Molecular Endocrinology, vol. 36, no. 3, pp. 435-448
    Contributions to Journals: Articles

  • A virus-neutralising antibody is not cytotoxic in vitro

    Kolb, A., Whitelaw, C. B. A.
    Molecular Immunology, vol. 43, no. 6, pp. 677-689
    Contributions to Journals: Articles

  • Studying human pathogens in animal models: Fine Tuning the Humanized Mouse

    Lassnig, C., Kolb, A., Strobl, B., Enjuanes, L., Muller, M.
    Transgenic Research, vol. 14, no. 6, pp. 803-806
    Contributions to Journals: Articles

  • Regulation of genes encoding proteolytic enzymes during mammary gland development

    Sorrell, D. A., Szymanowska, M., Boutinaud, M., Robinson, C., Clarkson, R. W. E., Stein, T., Flint, D. J., Kolb, A.
    Journal of Dairy Research, vol. 72, no. 4, pp. 433-441
    Contributions to Journals: Articles

  • Targeted modification of mammalian genomes

    Sorrell, D. A., Kolb, A.
    Biotechnology Advances, vol. 23, no. 7-8, pp. 431-469
    Contributions to Journals: Literature Reviews

  • Insulin-like growth factor binding proteins initiate cell death and extracellular matrix remodeling in the mammary gland

    Flint, D. J., Boutinaud, M., Tonner, E., Wilde, C. J., Hurley, W., Accorsi, P. A., Kolb, A., Whitelaw, C. B. A., Beattie, J., Allan, G. J.
    DOMESTIC ANIMAL ENDOCRINOLOGY, vol. 29, no. 2, pp. 274-282
    Contributions to Journals: Articles

  • Site-directed genome modification: derivatives of DNA-modifying enzymes as targeting tools

    Coates, C. J., Kaminski, J. M., Summers, J. B., Segal, D. J., Miller, A. D., Kolb, A.
    Trends in Biotechnology, vol. 23, no. 8, pp. 407-419
    Contributions to Journals: Literature Reviews

  • Site-directed genome modification: nucleic acid and protein modules for targeted integration and gene correction

    Kolb, A. F., Coates, C. J., Kaminski, J. M., Summers, J. B., Miller, A. D., Segal, D. J.
    Trends in Biotechnology, vol. 23, no. 8, pp. 399-406
    Contributions to Journals: Literature Reviews

  • Hormone and growth factor regulation of tissue remodeling in the mammary gland

    Flint, D., Allan, G., Beattie, J., Travers, M., Barber, M., Kolb, A., Whitelaw, C., Boutinaud, M., Binart, N., Kelly, P.
    Journal of Dairy Science, vol. 88, pp. 208
    Contributions to Journals: Articles

  • The first intron of the murine beta-casein gene contains a functional promoter

    Kolb, A.
    Biochemical and Biophysical Research Communications, vol. 306, no. 4, pp. 1099-1105
    Contributions to Journals: Articles

  • Structure and regulation of the murine gamma-casein gene

    Kolb, A.
    Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, vol. 1579, no. 2-3, pp. 101-116
    Contributions to Journals: Articles

  • Engineering immunity in the mammary gland

    Kolb, A.
    Journal of Mammary Gland Biology and Neoplasia, vol. 7, no. 2, pp. 123-134
    Contributions to Journals: Articles

  • Genome engineering using site-specific recombinases

    Kolb, A.
    Cloning and Stem Cells, vol. 4, no. 1, pp. 65-80
    Contributions to Journals: Articles

  • The prospects of modifying the antimicrobial properties of milk

    Kolb, A.
    Biotechnology Advances, vol. 19, no. 4, pp. 299-316
    Contributions to Journals: Literature Reviews

  • Selection-marker-free modification of the murine beta-casein gene using a lox2722 site

    Kolb, A.
    Analytical Biochemistry, vol. 290, no. 2, pp. 260-271
    Contributions to Journals: Articles

  • Virus-neutralizing monoclonal antibody expressed in milk of transgenic mice provides full protection against virus-induced encephalitis

    Kolb, A., Pewe, L., Webster, J., Perlman, S., Whitelaw, C. B. A., Siddell, S. G.
    Journal of General Virology, vol. 75, no. 6, pp. 2803-2809
    Contributions to Journals: Articles

  • A virus-neutralising monoclonal antibody expressed in the milk of transgenic mice

    Kolb, A., Webster, J., Whitelaw, C. B. A., Siddell, S. G.
    Advances in Experimental Medicine and Biology, vol. 494, pp. 411-414
    Contributions to Journals: Articles

  • Insertion of a foreign gene into the beta-casein locus by Cre-mediated site-specific recombination

    Kolb, A., Ansell, R., McWhir, J., Siddell, S. G.
    Gene, vol. 227, no. 1, pp. 21-31
    Contributions to Journals: Articles

  • A functional eukaryotic promoter is contained within the first intron of the hGH-N coding region

    Kolb, A., Gunzburg, W. H., Brem, G., Erfle, V., Salmons, B.
    Biochemical and Biophysical Research Communications, vol. 247, no. 2, pp. 332-337
    Contributions to Journals: Articles

  • Characterization of determinants involved in the feline infectious peritonitis virus receptor function of feline aminopeptidase N

    Hegyi, A., Kolb, A.
    Journal of General Virology, vol. 79, no. 6, pp. 1387-1391
    Contributions to Journals: Articles

  • Isolation and recombinant expression of an MHV-JHM neutralising monoclonal antibody

    Kolb, A., Lechermaier, M., Heister, A., Toksoy, A., Siddell, S. G.
    Advances in Experimental Medicine and Biology, vol. 440, pp. 657-664
    Contributions to Journals: Articles

  • Molecular analysis of the coronavirus-receptor function of aminopeptidase N

    Kolb, A., Hegyi, A., Maile, J., Heister, A., Hagemann, M., Siddell, S. G.
    Advances in Experimental Medicine and Biology, vol. 440, pp. 61-67
    Contributions to Journals: Articles

  • Genomic targeting of a bicistronic DNA fragment by Cre-mediated site-specific recombination

    Kolb, A., Siddell, S. G.
    Gene, vol. 203, no. 2, pp. 209-216
    Contributions to Journals: Articles

  • Identification of residues critical for the human coronavirus 229E receptor function of human aminopeptidase N

    Kolb, A., Hegyi, A., Siddell, S. G.
    Journal of General Virology, vol. 78, no. 11, pp. 2795-2802
    Contributions to Journals: Articles

  • Expression of a recombinant monoclonal antibody from a bicistronic mRNA

    Kolb, A., Siddell, S. G.
    Hybridoma, vol. 16, no. 5, pp. 421-426
    Contributions to Journals: Articles

  • Genomic targeting with an MBP-Cre fusion protein

    Kolb, A., Siddell, S. G.
    Gene, vol. 183, no. 1-2, pp. 53-60
    Contributions to Journals: Articles

  • Characterization of functional domains in the human coronavirus HCV 229E receptor

    Kolb, A., Maile, J., Heister, A., Siddell, S. G.
    Journal of General Virology, vol. 77, no. 10, pp. 2515-2521
    Contributions to Journals: Articles

  • Characterization of a protein that binds a negative regulatory element in the mammary-specific whey acidic protein promoter

    Kolb, A., Albang, R., Brem, G., Erfle, V., Gunzburg, W. H., Salmons, B.
    Biochemical and Biophysical Research Communications, vol. 217, no. 3, pp. 1045-1052
    Contributions to Journals: Articles

  • Negative Regulatory Element in the Mammary Specific Whey Acidic Protein Promoter

    Kolb, A., GUNZBURG, W. H., ALBANG, R., BREM, G., ERFLE, V., SALMONS, B.
    Journal of Cellular Biochemistry, vol. 56, no. 2, pp. 245-261
    Contributions to Journals: Articles

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