Senior Research Fellow
- About
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- Email Address
- a.kolb@abdn.ac.uk
- Telephone Number
- +44 (0)1224 438645
- School/Department
- School of Medicine, Medical Sciences and Nutrition
Biography
2015-present: Senior Research Fellow, Rowett Institute, University of Aberdeen, Scotland, UK.
2006-2015: Research Fellow, Rowett Institute, University of Aberdeen, Scotland, UK.
2002-2005: Principal Investigator, Molecular Recognition Group, Hannah Research Institute, Ayr, Scotland, UK.
1997-2002: Investigator, Cell Physiology Group, Hannah Research Institute, Ayr, Scotland, UK.
1992-1997: Postdoc, Institute of Virology and Immunology, University of Würzburg, Germany.
1989-1992: PhD Student, Institute of Molecular Animal Breeding, University of Munich, Germany.
Memberships and Affiliations
- Internal Memberships
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Postgraduate Research Coordinator, Rowett Institute
Chair, PGR Liaison Committee, School of Medicine Medical Sciences and Nutrition
- External Memberships
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Associate Fellow of The Higher Education Academy
- Research
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Research Overview
My research interest covers two overlapping research areas, namely:
- Impact of secondary plant metabolites and toxins on metabolic health regulation, mediated by the gut microbiome and bile acid
- Impact of nutrition in early life stages on adult metabolic health and aging.
Research Areas
Accepting PhDs
I am currently accepting PhDs in Nutrition and Health.
Please get in touch if you would like to discuss your research ideas further.
Current Research
Dietary and environmental impacts on metabolic health
The gut represents the main interface with which mammals interact with their environment. Bile acids are essential for the emulsification and uptake of dietary lipids and lipid-soluble vitamins. However, bile acids also act as signaling molecules via several nuclear receptors and G-protein coupled receptors. Many of these signaling events take place in the gut, but bile acids also circulate in serum and can also signal in other organs. Bile acids are synthesized in the liver, secreted via the gall bladder, and chemically modified by the gut microbiome to give rise to more than 20 different bile acids. We have found that dietary components can substantially alter the composition of bile acids in the gut and in serum. Environmental toxins (including mycotoxins and synthetic plastic compounds) can interact with bile acid signaling (directly or via the modulation of the microbiome) and derail the metabolic equilibrium. We are investigating the molecular mechanisms which are critical for bile acid signaling and how dietary components and toxins can influence these events. We apply this knowledge to develop cell-based assay systems to measure toxin concentrations in environmental and food samples.
Early life nutrition and metabolic health outcomes
Nutrition during early phases of life can have profound impacts on metabolic health in adulthood. Intrauterine growth restriction has been shown to alter body composition and epigenetic processes. This leads to increased susceptibility to obesity, type II diabetes and cardiovascular disease and shortened lifespan. Attenuated growth after birth, in contrast, is protective against aspects of the metabolic syndrome and extends lifespan. We are using in vitro and in vivo models of post-natal attenuated growth to study the molecular events which underlie the improved metabolic health outcomes and delayed aging. We apply this knowledge to develop pharmacological interventions to improve metabolic health.
Supervision
My current supervision areas are: Nutrition and Health.
Current PhD students:
Khulod Hasaballah
Amane Alaroud
Amanda Mathieson
Brendan Kesler
Past PhD students:
Alina Zitskaja (2016-2021)
Patrikas Pultinevicius (2015-2020)
Jennifer Harbottle (2013-2017)
Christopher Knowles (2012-2016)
- Teaching
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Teaching Responsibilities
ME2511: Student Selected Components Year 1, course coordinator
SM2501: Research Skills for Medical Sciences
SM2001: Foundation Skills for Medical Sciences
GN3502: Genetics
Non-course Teaching Responsibilities
Supervision of Honours and MSc projects
- Publications
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Page 1 of 1 Results 1 to 50 of 50
Maternal α-casein deficiency extends the lifespan of offspring and programmes their body composition
GeroScienceContributions to Journals: Articles- [ONLINE] DOI: https://doi.org/10.1007/s11357-024-01273-2
Site specific insertion of a transgene into the murine α-casein (CSN1S1) gene results in the predictable expression of a recombinant protein in milk
Biotechnology Journal, vol. 19, no. 2, e2300287Contributions to Journals: ArticlesThe human genome at 20: how biology’s most-hyped breakthrough led to anticlimax and arrests
Contributions to Specialist PublicationsA cell-based assay system for activators of the environmental cell stress response
Analytical Biochemistry, vol. 592, 113583Contributions to Journals: ArticlesAntiobesity functional leads and targets for drug development
Phytochemicals as Lead Compounds for New Drug Discovery. Egbuna, C., Kumar, S., Ifemeje, J., Ezzat, S., Kaliyaperumal, S. (eds.). Elsevier, pp. 143-160, 18 pagesChapters in Books, Reports and Conference Proceedings: Chapters- [ONLINE] DOI: https://doi.org/10.1016/B978-0-12-817890-4.00009-3
- [ONLINE] View publication in Scopus
Folate deficiency promotes differentiation of vascular smooth muscle cells without affecting the methylation status of regulated genes
Biochemical Journal, vol. 476, no. 19, pp. 2769-2795Contributions to Journals: Articles- [ONLINE] DOI: https://doi.org/10.1042/BCJ20190275
- [OPEN ACCESS] http://aura.abdn.ac.uk/bitstream/2164/15140/1/BCJ_Folate_deficiency_Kolb_et_al_AAM.pdf
- [ONLINE] View publication in Scopus
Recombinase-Mediated Cassette Exchange Using Adenoviral Vectors
Methods in Molecular Biology, vol. 1642, pp. 127-150Contributions to Journals: Articles- [ONLINE] DOI: https://doi.org/10.1007/978-1-4939-7169-5_9
Using the CRISPR/Cas9 system to understand neuropeptide biology and regulation
Neuropeptides, vol. 64, pp. 19-25Contributions to Journals: ArticlesCD13/aminopeptidase N is a negative regulator of mast cell activation
The FASEB Journal, vol. 30, no. 6, pp. 2225-2235Contributions to Journals: Articles- [ONLINE] DOI: https://doi.org/10.1096/fj.201600278
Genetics and biosynthesis of milk proteins
Advanced Dairy Chemistry: Volume 1A: Proteins: Basic Aspects, 4th Edition. McSweeny, P. L. H., Fox, P. F. (eds.). Springer US, pp. 431-461, 31 pagesChapters in Books, Reports and Conference Proceedings: Chapters- [ONLINE] DOI: https://doi.org/10.1007/978-1-4614-4714-6_14
- [ONLINE] View publication in Scopus
Folate deficiency enhances the inflammatory response of macrophages
Molecular Immunology, vol. 54, no. 2, pp. 164-172Contributions to Journals: Articles- [ONLINE] DOI: https://doi.org/10.1016/j.molimm.2012.11.012
Behaviour of postnatally growth-impaired mice during malnutrition and after partial weight recovery
Nutritional Neuroscience, vol. 16, no. 3, pp. 125-134Contributions to Journals: Articles- [ONLINE] DOI: https://doi.org/10.1179/1476830512Y.0000000038
Mammary gland development is delayed in mice deficient for aminopeptidase N
Transgenic Research, vol. 22, no. 2, pp. 425-434Contributions to Journals: Articles- [ONLINE] DOI: https://doi.org/10.1007/s11248-012-9654-7
Milk lacking α-casein leads to permanent reduction in body size in mice
PloS ONE, vol. 6, no. 7, e21775Contributions to Journals: Articles- [ONLINE] DOI: https://doi.org/10.1371/journal.pone.0021775
- [OPEN ACCESS] http://aura.abdn.ac.uk/bitstream/2164/3568/1/Milk_lacking.pdf
Zinc finger recombinases with adaptable DNA sequence specificity
PloS ONE, vol. 6, no. 4, e19537Contributions to Journals: Articles- [ONLINE] DOI: https://doi.org/10.1371/journal.pone.0019537
- [OPEN ACCESS] http://aura.abdn.ac.uk/bitstream/2164/3628/1/Zinc_finger.pdf
TRANSFER VEHICLE FOR PRODUCING PROTEINS IN TRANSGENIC ANIMALS
Patents: PatentsRecombinase mediated cassette exchange into genomic targets using an adenovirus vector
Nucleic Acids Research, vol. 38, no. 11, e123Contributions to Journals: Articles- [ONLINE] DOI: https://doi.org/10.1093/nar/gkq192
Hyperglycaemia: effects on expression of genes affecting epithelial differentiation and vascularisation
Scottish Medical Journal, vol. 54, no. 4, pp. 48Contributions to Journals: Abstracts- [ONLINE] DOI: https://doi.org/10.1258/rsmsmj.54.4.47
Analysis of mammary specific gene locus regulation in differentiated cells derived by somatic cell fusion
Experimental Cell Research, vol. 315, no. 3, pp. 508-522Contributions to Journals: Articles- [ONLINE] DOI: https://doi.org/10.1016/j.yexcr.2008.10.029
Hyperglycaemia: Effect on Expression of Genes Affecting Epithelial Differentiation and Vascularisation
Scottish Medical Journal, vol. 54, no. 1, pp. 52Contributions to Journals: Abstracts- [ONLINE] DOI: https://doi.org/10.1258/rsmsmj.54.1.51
EMMPRIN (basigin/CD147) Expression is not Correlated With MMP Activity During Adult Mouse Mammary Gland Development
Journal of Cellular Biochemistry, vol. 106, no. 1, pp. 52-62Contributions to Journals: Articles- [ONLINE] DOI: https://doi.org/10.1002/jcb.21975
Prolactin inhibits cell loss and decreases matrix metalloproteinase expression in the involuting mouse mammary gland but fails to prevent cell loss in the mammary glands of mice expressing IGFBP-5 as a mammary transgene
Journal of Molecular Endocrinology, vol. 36, no. 3, pp. 435-448Contributions to Journals: Articles- [ONLINE] DOI: https://doi.org/10.1677/jme.1.01873
A virus-neutralising antibody is not cytotoxic in vitro
Molecular Immunology, vol. 43, no. 6, pp. 677-689Contributions to Journals: Articles- [ONLINE] DOI: https://doi.org/10.1016/j.molimm.2005.04.008
Studying human pathogens in animal models: Fine Tuning the Humanized Mouse
Transgenic Research, vol. 14, no. 6, pp. 803-806Contributions to Journals: Articles- [ONLINE] DOI: https://doi.org/10.1007/s11248-005-1676-y
Regulation of genes encoding proteolytic enzymes during mammary gland development
Journal of Dairy Research, vol. 72, no. 4, pp. 433-441Contributions to Journals: Articles- [ONLINE] DOI: https://doi.org/10.1017/S0022029905001202
Targeted modification of mammalian genomes
Biotechnology Advances, vol. 23, no. 7-8, pp. 431-469Contributions to Journals: Literature Reviews- [ONLINE] DOI: https://doi.org/10.1016/j.biotechadv.2005.03.003
Insulin-like growth factor binding proteins initiate cell death and extracellular matrix remodeling in the mammary gland
DOMESTIC ANIMAL ENDOCRINOLOGY, vol. 29, no. 2, pp. 274-282Contributions to Journals: Articles- [ONLINE] DOI: https://doi.org/10.1016/j.domaniend.2005.02.021
Site-directed genome modification: derivatives of DNA-modifying enzymes as targeting tools
Trends in Biotechnology, vol. 23, no. 8, pp. 407-419Contributions to Journals: Literature Reviews- [ONLINE] DOI: https://doi.org/10.1016/j.tibtech.2005.06.009
Site-directed genome modification: nucleic acid and protein modules for targeted integration and gene correction
Trends in Biotechnology, vol. 23, no. 8, pp. 399-406Contributions to Journals: Literature Reviews- [ONLINE] DOI: https://doi.org/10.1016/j.tibtech.2005.06.005
Hormone and growth factor regulation of tissue remodeling in the mammary gland
Journal of Dairy Science, vol. 88, pp. 208Contributions to Journals: ArticlesThe first intron of the murine beta-casein gene contains a functional promoter
Biochemical and Biophysical Research Communications, vol. 306, no. 4, pp. 1099-1105Contributions to Journals: Articles- [ONLINE] DOI: https://doi.org/10.1016/S0006-291X(03)01104-5
Structure and regulation of the murine gamma-casein gene
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, vol. 1579, no. 2-3, pp. 101-116Contributions to Journals: ArticlesEngineering immunity in the mammary gland
Journal of Mammary Gland Biology and Neoplasia, vol. 7, no. 2, pp. 123-134Contributions to Journals: Articles- [ONLINE] DOI: https://doi.org/10.1023/A:1020395701887
Genome engineering using site-specific recombinases
Cloning and Stem Cells, vol. 4, no. 1, pp. 65-80Contributions to Journals: Articles- [ONLINE] DOI: https://doi.org/10.1089/153623002753632066
The prospects of modifying the antimicrobial properties of milk
Biotechnology Advances, vol. 19, no. 4, pp. 299-316Contributions to Journals: Literature Reviews- [ONLINE] DOI: https://doi.org/10.1016/S0734-9750(01)00069-6
Selection-marker-free modification of the murine beta-casein gene using a lox2722 site
Analytical Biochemistry, vol. 290, no. 2, pp. 260-271Contributions to Journals: Articles- [ONLINE] DOI: https://doi.org/10.1006/abio.2000.4984
Virus-neutralizing monoclonal antibody expressed in milk of transgenic mice provides full protection against virus-induced encephalitis
Journal of General Virology, vol. 75, no. 6, pp. 2803-2809Contributions to Journals: Articles- [ONLINE] DOI: https://doi.org/10.1128/JVI.75.6.2803-2809.2001
A virus-neutralising monoclonal antibody expressed in the milk of transgenic mice
Advances in Experimental Medicine and Biology, vol. 494, pp. 411-414Contributions to Journals: ArticlesInsertion of a foreign gene into the beta-casein locus by Cre-mediated site-specific recombination
Gene, vol. 227, no. 1, pp. 21-31Contributions to Journals: Articles- [ONLINE] DOI: https://doi.org/10.1016/S0378-1119(98)00607-6
A functional eukaryotic promoter is contained within the first intron of the hGH-N coding region
Biochemical and Biophysical Research Communications, vol. 247, no. 2, pp. 332-337Contributions to Journals: ArticlesCharacterization of determinants involved in the feline infectious peritonitis virus receptor function of feline aminopeptidase N
Journal of General Virology, vol. 79, no. 6, pp. 1387-1391Contributions to Journals: ArticlesIsolation and recombinant expression of an MHV-JHM neutralising monoclonal antibody
Advances in Experimental Medicine and Biology, vol. 440, pp. 657-664Contributions to Journals: ArticlesMolecular analysis of the coronavirus-receptor function of aminopeptidase N
Advances in Experimental Medicine and Biology, vol. 440, pp. 61-67Contributions to Journals: ArticlesGenomic targeting of a bicistronic DNA fragment by Cre-mediated site-specific recombination
Gene, vol. 203, no. 2, pp. 209-216Contributions to Journals: Articles- [ONLINE] DOI: https://doi.org/10.1016/S0378-1119(97)00515-5
Identification of residues critical for the human coronavirus 229E receptor function of human aminopeptidase N
Journal of General Virology, vol. 78, no. 11, pp. 2795-2802Contributions to Journals: ArticlesExpression of a recombinant monoclonal antibody from a bicistronic mRNA
Hybridoma, vol. 16, no. 5, pp. 421-426Contributions to Journals: ArticlesGenomic targeting with an MBP-Cre fusion protein
Gene, vol. 183, no. 1-2, pp. 53-60Contributions to Journals: ArticlesCharacterization of functional domains in the human coronavirus HCV 229E receptor
Journal of General Virology, vol. 77, no. 10, pp. 2515-2521Contributions to Journals: ArticlesCharacterization of a protein that binds a negative regulatory element in the mammary-specific whey acidic protein promoter
Biochemical and Biophysical Research Communications, vol. 217, no. 3, pp. 1045-1052Contributions to Journals: Articles- [ONLINE] DOI: https://doi.org/10.1006/bbrc.1995.2875
Negative Regulatory Element in the Mammary Specific Whey Acidic Protein Promoter
Journal of Cellular Biochemistry, vol. 56, no. 2, pp. 245-261Contributions to Journals: Articles