Professor Anne Donaldson
BA (University of Cambridge, 1989), PhD (MRC Laboratory of Molecular Biology, Cambridge, 1994)
Room 2:17 Institute of Medical Sciences Foresterhill University of Aberdeen Aberdeen AB25 2ZD Lab phone +44 (0)1224 437312
Anne Donaldson studied Natural Sciences at the University of Cambridge, completed her PhD at the MRC Laboratory of Molecular Biology, then moved as a NATO/SERC postdoctoral fellow to the University of Washington in Seattle. Anne established her lab as a Royal Society University Research Fellow at the University of Dundee, and in 2001 Anne was chosen as an EMBO Young Investigator. The Donaldson laboratory has been at the University of Aberdeen Institute of Medical Sciences since 2003. The lab is funded by Cancer Research UK and the BBSRC.
Anne teaches on several Aberdeen University Molecular & Cell Biology courses, and from 2006-13 was Examinations Officer & Chair of Examinations Boards (Molecular & Cell Biology). Anne was an Organizer of the Cold Spring Harbor Eukaryotic DNA Replication & Genome Maintenance meeting from 2014-2019, and currently serves as Vice-President for External Relations for the Genetics Society.
Oct 2021: We have Postdoc and PhD positions available in the lab to begin in 2022, funded by Cancer Research UK. If you are interested please send CV to Anne Donaldson, who is pleased to hear from potential candidates.
Human cells contain 1.8 metres of DNA in a nucleus only about 6 microns in diameter. During chromosome replication this entire length of DNA must be duplicated exactly once with perfect accuracy, so that the strands can be disentangled and precisely segregated to the daughter cells. The DNA is extremely vulnerable to damage during this process, and cells must deal with thousands of potentially lethal DNA damage events every single day. Members of the Donaldson lab investigate the controls over DNA replication and damage repair. Understanding chromosome maintenance will suggest new therapeutic strategies in the fight against cancer, as well as illuminating the basic mechanisms at the heart of the cell division cycle.
The budding yeast S. cerevisiae provides an excellent model organism for studying the fundamentals of chromosome biology, because of the remarkable molecular genetics tools available for this system. DNA replication initiates at multiple sites on each chromosome called replication origins. We use molecular genetics to understand the processes of yeast DNA replication, which we then investigate in human cells. Using this approach we have discovered several molecular mechanisms of replication control that operate throughout eukaryotic cells.
Our focus of interest is understanding the molecular machinery controlling origin initiation, replication fork progression, and chromosome maintenance. We use a combination of advanced proteomic, genomic and microscopy methods to investigate the cellular components that regulate these DNA replication and repair processes.
Funding and Grants
Cancer Research UK Programme Award (£1,561,000) ‘How does Rif1 regulate DNA replication and cell recovery after chemotherapeutic replication inhibition?' Grant to Prof Anne Donaldson & Dr Shin-ichiro Hiraga
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Protein Phosphatases and DNA Replication InitiationThe Initiation of DNA Replication in Eukaryotes. Kaplan, D. L. (ed.). Springer, pp. 461-477, 17 pagesChapters in Books, Reports and Conference Proceedings: Chapters
Replication-Coupled PCNA Unloading by the Elg1 Complex Occurs Genome-wide and Requires Okazaki Fragment LigationCell Reports, vol. 12, no. 5, pp. 774-787Contributions to Journals: Articles
At Short Telomeres Tel1 Directs Early Replication and Phosphorylates Rif1PLoS Genetics, vol. 10, no. 10, e1004691Contributions to Journals: Articles
Rif1 controls DNA replication by directing Protein Phosphatase 1 to reverse Cdc7-mediated phosphorylation of the MCM complexGenes & Development, vol. 28, no. 4, pp. 372-383Contributions to Journals: Articles
Negative regulation of DNA replication by Rif1 through protein phosphatase PP-1CSH Meeting "Eukaryotic DNA replication and genome maintenance"Contributions to Conferences: Posters
Is PCNA unloading the central function of the Elg1/ATAD5 replication factor C-like complex?Cell Cycle, vol. 12, no. 16, pp. 2570-2579Contributions to Journals: Articles
The Elg1 replication factor C-like complex functions in PCNA unloading during DNA replicationMolecular Cell, vol. 50, no. 2, pp. 273-280Contributions to Journals: Articles
Negative regulation of DNA replication by Rif1 through DDKBritish Yeast Group Meeting 2013Contributions to Conferences: Posters
TFIIIC Localizes Budding Yeast ETC Sites to the Nuclear PeripheryMolecular Biology of the Cell, vol. 23, no. 14, pp. 2741-2754Contributions to Journals: Articles
Quantitative proteomic analysis of yeast DNA replication proteinsMethods, vol. 57, no. 2, pp. 196-202Contributions to Journals: Articles