Quantitative Imaging Flow Cytometry using “Amnis ImageStream-X MarkII”

The Amnis ImageStreamX MKII Imaging Flow Cytometer combines uniquely detailed microscopic imaging of individual cells with the speed, sensitivity, quantification and multi-parameter phenotyping abilities of flow cytometry. The ImageStream produces multiple high resolution images of every cell directly in flow using brightfield, darkfield and multiple fluorescent markers, with a sensitivity that exceeds conventional flow cytometers.

This unique combination of the ImageStreamX MKII is designed for a wide range of applications in biological and medical research including:

  • Cell signaling
  • Nuclear translocation studies
  • Internalization of ligands,
  • Phagocytosis, pinocytosis
  • Shape change (chemokine-induced, etc)
  • Cell-Cell interactions, including immune synapse studies
  • Apoptosis and autophagy, including LC3 spot counting
  • Radiation repair, including γ -H2AX spot counting
  • Intracellular trafficking and organelle co-localization
  • Cell cycle and mitosis studies in heterogeneous populations
  • Sensitive FISH for rare events
  • Morphology-based cell classification

Read more about the Amnis ImageStream X MarkII

Preclinical Imaging using "In Vivo MS FX Pro"

Preclinical ImagingOne system Powerful Multispectral In vivo Imaging modality includes:

  • Fluorescence (multi-wavelength)
  • Luminescence
  • Radioisotopic and
  • X-ray imaging

MS FX PRO brings the broadest range of imaging applications - from protein blots to complex, longitudinal studies of disease states in vivo.

Read more about the In Vivo MS FX Pro

Bio-contained Single Cell Isolation using Influx

  • 5 laser, 15 Fluorescence, 3 scatter, polarization and small particle detection unit
  • Multi-way sorting; up to 6 different populations simultaneously
  • High-Speed sorting into wide range of collection vehicles
  • BSL-II biosafety cabinet for biohazardous samples

Powerful high-speed single event sorter adapts to your needs for

  • Single Cell Genomics (SCG)
  • Micro-Particle Research
  • Environmental & Plant cytometry
  • Microbiology and Marine Biology

Read more about the BD Influx BSLII Sorter

Multi-Parameter Flow Cytometry on Single Cell Level

IFCC provides high-end Multi-Laser instruments (including YG laser) for up to 20 Parameters, for example: Intracellular Phospho Cell Signaling

  • Analyse phosphorylation at a single cell level in heterogeneous complex populations
  • Correlate multiple markers simultaneously, e.g. CD markers together with pSTAT molecules
  • Direct Analysis of rare cell types, i.e. DC or Stem Cells

Read more about: BD Calibur | BD Fortessa | BD LSR II | BD FACSArray

Multi-Plex Analysis

Measure multiple analytes of interest in a single run/cycle of the multiplex Assay with Luminex 200:

  • Up to 100 different analytes (e.g. cytokines) in small volume of sample (25-50)

Generates more reproducible data quickly for

  • Multiple Cell signalling molecules
  • miRNA Expression
  • Biomarker discovery
  • Clinical disease marker (e.g. CVD or RA)

Read more about the BioPlex 200

Additional Instruments for sample processing

Save time and don't wait any longer for reproducibility! IFCC is offering supportive instruments for standardizing your single cell research:

  • gentleMACS for fully automated tissue dissociation into single-cell suspension
  • Z2 Coulter Counter for automated counting and sizing your single-cell suspension

Time-saving - Reliability - Versatility!

Read more about: gentleMACS | Z2 Coulter Counter