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Mr Gary Duncan

Mr Gary Duncan
Mr Gary Duncan
Mr Gary Duncan

Research Technician

About
Email Address
gary.duncan@abdn.ac.uk
Telephone Number
+44 (0)1224 438669
School/Department
School of Medicine, Medical Sciences and Nutrition

Biography

Gary has over 25 years of Mass Spectrometry experience.  He started work at what was then the Rowett Research Institute in 1986, and after six weeks training in analytical techniques, started working in the Gas Isotope Ratio Mass Spectrometry (GIRMS) laboratory assisting Dr Brian McGaw and Mr Eric Milne. As interest in this technique grew, the lab eventually had four mass spectrometers dedicated to analysing the stable isotopes of Carbon, Hydrogen and Oxygen from a variety of biological sources, supporting research into energy expenditure and protein synthesis. The research programmes of the Rowett then required Liquid Chromatography Mass Spectrometry (LC/MS) and a MAT 900 double-sector LC-MS/MS system was purchased in 1998, along with a LaserMAT MALDI mass spectrometer. Initially his role was to develop techniques for sample analysis on the MALDI, but soon took over the role of managing the LC/MS mass facility. This coincided with a ‘take-off’ in the popularity of using LCMSMS in sample analysis, firstly in the growth of identifying proteins separated on 2D gels and more recently the targeted quantitation analysis of small molecules. The main areas of analysis are in the quantification of Polyphenols relating to dietary consumption and human health and the quantification of Mycotoxins in foods and crops, in particular, the detoxification of Deoxynivalenol. The LCMSMS facility now has four mass spectrometers, with access to an ion trap to support work in these areas of research.

Other Areas of interest include:

  • Vitamin D3
  • Methylamines
  • Anthocyanins
  • Nicotinamides
  • Carboxymethyl Lysines
  • Antibiotics

Latest Publications

View My Publications

Publications

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  • Identification of potential serum biomarkers of inflammation and lipid modulation that are altered by fish oil supplementation in healthy volunteers

    De Roos, B., Geelen, A., Ross, K., Rucklidge, G., Reid, M., Duncan, G., Caslake, M., Horgan, G., Brouwer, I. A.
    Proteomics, vol. 8, no. 10, pp. 1965-1974
    Contributions to Journals: Articles

  • Disruption of lipid metabolism in the liver of the pregnant rat fed folate-deficient and methyl donor-deficient diets

    McNeil, C. J., Hay, S., Rucklidge, G. J., Reid, M. D., Duncan, G. J., Maloney, C. A., Rees, W.
    British Journal of Nutrition, vol. 99, no. 2, pp. 262-271
    Contributions to Journals: Articles

  • Profiling of mitochondrial associated proteins from rat colon

    Padidar, S., Bestwick, C., King, T. P., Rucklidge, G. J., Duncan, G. J., Reid, M. D., Drew, J.
    Journal of Cellular Biochemistry, vol. 103, no. 1, pp. 78-97
    Contributions to Journals: Articles

  • The effects of fish oil and DHA on soluble epoxide hydrolase in apoE-/- mice

    Mavrommatis, Y. I., Thies, F., Ross, K., Gordon, M. J., McLeod, E., Sneddon, A. A., Rucklidge, G. J., Reid, M. D., Duncan, G. J., Mayer, C., Horgan, G., Arthur, J., De Roos, B.
    Proceedings of the Nutrition Society, vol. 66, no. Supplement OCA-B , 103A
    Contributions to Journals: Abstracts

  • Extra virgin oil decreases atherosclerosis despite increased hepatic fat accumulation in apolipoprotein E knockout mice: A proteomics approach

    Arbones-Mainar, J., Ross, K., Rucklidge, G. J., Reid, M. D., Duncan, G. J., Arthur, J., Horgan, G., Navarro, M. A., Charnicer, R., Arnal, C., Osada, J., De Roos, B.
    Diabetes and Vascular Disease Research, vol. 4, no. 1 Suppl. , S189
    Contributions to Journals: Abstracts

  • Biochemical and proteomic changes in the colon and liver of rats in response to long-term folate deficiency

    Duthie, S. J., Grant, G., Rucklidge, G. J., Reid, M. D., Duncan, G. J., Pirie, L. P., Horgan, G.
    Proceedings of the Nutrition Society, vol. 66, no. Suppl. OCA-B, pp. 111A
    Contributions to Journals: Abstracts

  • Extra Virgin Olive Oils Increase Hepatic Fat Accumulation and Hepatic Antioxidant Protein Levels in APOE-/- Mice

    Arbones-Mainar, J. M., Ross, K., Rucklidge, G. J., Reid, M. D., Duncan, G. J., Arthur, J., Horgan, G. W., Navarro, M. A., Carnicer, R., Arnal, C., Osada, J., De Roos, B.
    Journal of Proteome Research, vol. 6, no. 10, pp. 4041-4054
    Contributions to Journals: Articles

  • Salicylate modulates oxidative stress in the rat colon: a proteomic approach

    Drew, J. E., Padidar, S., Horgan, G., Duthie, G. G., Russell, W. R., Reid, M., Duncan, G., Rucklidge, G. J.
    Biochemical Pharmacology, vol. 72, no. 2, pp. 204-216
    Contributions to Journals: Articles

  • A novel class of CoA-transferase involved in short-chain fatty acid metabolism in butyrate-producing human colonic bacteria

    Charrier, C., Duncan, G. J., Reid, M. D., Rucklidge, G. J., Henderson, D., Young, P., Russell, V. J., Aminov, R. I., Flint, H. J., Louis, P.
    Microbiology , vol. 152, no. 1, pp. 179-185
    Contributions to Journals: Articles

  • Divergent mechanisms of cis9, trans11-and trans10, cis12-conjugated linoleic acid affecting insulin resistance and inflammation in apolipoprotein E knockout mice: a proteomics approach

    De Roos, B., Rucklidge, G. J., Reid, M. D., Ross, K., Duncan, G. J., Navarro, M. A., Arbones-Mainar, J. M., Guzman-Garcia, M. A., Osada, J., Browne, J., Loscher, C. E., Roche, H. M.
    The FASEB Journal, vol. 19, no. 9, pp. 1746-1767
    Contributions to Journals: Articles

  • A proteomics approach to identify changes in protein profiles in pre-cancerous colon

    Drew, J., Rucklidge, G. J., Duncan, G., Lufty, A., Farquharson, A. J., Reid, M. D., Russell, W. R., Morrice, P. C., Arthur, J. R., Duthie, G. G.
    Biochemical and Biophysical Research Communications, vol. 330, no. 1, pp. 81-87
    Contributions to Journals: Articles

  • Rat metallothionein-2 contains N-alpha-acetylated and unacetylated isoforms

    Beattie, J. H., Wood, A., Duncan, G. J.
    Electrophoresis, vol. 20, no. 7, pp. 1613-1618
    Contributions to Journals: Articles

  • The Influence of Exercise on the Energy Requirements of Adult Males in the UK

    Haggarty, P., McNeill, G., Manneh, M. K., Davidson, L., Milne, E., Duncan, G., Ashton, J.
    British Journal of Nutrition, vol. 72, no. 6, pp. 799-813
    Contributions to Journals: Articles

  • A Rapid Method for the Preparation of Combustion Samples for Stable Carbon Isotope Analysis by Isotope Ratio Mass-Spectrometry

    MCGAW, B. A., Milne, E., Duncan, G. J.
    Biomedical & Environmental Mass Spectrometry, vol. 16, no. 1-12, pp. 269-273
    Contributions to Journals: Articles
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