Last modified: 22 Oct 2021 09:20
An exciting course that brings a student right up to date with bioinformatics, genome assembly and annotation technology. You, the student, will isolate a novel bacterium from the environment and purify its DNA. This will be whole genome sequenced, and you will then assemble your own bacterial genome and functionally annotate it, taught by expert bioinformaticians from our Centre for Genome-Enabled Biology and Medicine. Your analysis will enable you to identify the species you have isolated, its metabolic and environmental responses, growth constraints and regulation, cell surface markers and antibiotic resistance, producing a full inventory of genes and their presumptive functions. Overall the course prepares a student for future employment in a biosciences workplace where genome sequencing and annotation is becoming an everyday tool of modern biotechnology.
|Session||Second Sub Session||Credit Points||15 credits (7.5 ECTS credits)|
This course will take students through the exciting and challenging process of analysing the sequence of a bacterial genome from an isolate that they themselves have identified and purified DNA from in semester 1. The course will teach students how to quality filter and trim raw sequencing data, assemble a genome, quality control the output, annotate genes, and carry out functional gene analysis using modern bioinformatics, to produce an assembled genome and full inventory of the genes of a novel bacterial isolate. This course will develop your knowledge and skills in bioinformatics analysis of genomic data and you will learn how to use scripting via the command line and the Galaxy interface.
Students wishing to assemble and annotate the genome of a bacterial isolate that they have isolated and extracted DNA from themselves must take Biotechnology BT5014, where this will be performed in a laboratory practical in semester 1. However, BT5014 is not a mandatory pre-requisite for this course and if you have not studied BT5014, bacterial genome raw sequencing reads will be provided to you to use for assembly and annotation in MB5522.
Information on contact teaching time is available from the course guide.
Coding test (short answer test) (15 %)
Short answer test (15 %)
Course blog (5 %)
3500 word Report (65 %)
Resit for students taking the course in AY20/21:
Resit (viva) will assess understanding of the learning outcomes of the course, does not cover individual assignments.
3 x MCQ – formative (0%)
|Knowledge Level||Thinking Skill||Outcome|
|Procedural||Evaluate||Assemble a bacterial genome using a web-based platform and using the command line, and evaluate assembly quality|
|Procedural||Create||Annotate a bacterial genome and use biological databases to gain insight into gene functions.|
|Conceptual||Apply||Understand Next Generation Sequencing (NGS) technologies and data, and its application|
|Procedural||Create||Develop analytical and critical skills in microbial genomics|
|Procedural||Analyse||Basic skills in Unix/Command line and using a High Performance Compute Cluster|