Fungal Infection & Diagnosis

Fungal infections and diagnosis – a two-day bespoke training course for all
WTSA-MMFI international students hosted by Dr Elizabeth Johnson and colleagues at the Public Health England Mycology Reference Laboratory in Bristol (run annually in September 2014, 2015 and scheduled for 2016).  The programme for the two workshop typically covers:

Role of Reference Laboratory: Brief overview of fungal infection    Elizabeth Johnston

Microscopy and culture of specimens and identification of common filamentous agents of deep fungal infection                         Andy Borman

Laboratory Session 1: Isolation and identification of moulds:                     

Demonstration of general techniques with opportunities to make mounts of systemic pathogens and superficial pathogens                                                                        Andy Borman and Chris Linton

Tour of dermatology set-up and microscopy Marian Houldsworth   

MALDI-ToF identification methods                   Mark Fraser

Laboratory Session II: Isolation and identification of yeast:

MALDI-ToF, Histology, germ tube – set-up, Cornmeal agar, CHROMagar, Indian ink stain, histology and antigen test for cryptococcosis                             Mark Fraser, Adrien Szekely and Elizabeth Johnston

Susceptibility testing yeast and moulds          Elizabeth Johnston         

Surrogate markers in the diagnosis of invasive fungal infection          Chris Linton

Laboratory Session III: Diagnosis of invasive fungal infection:

Galactomannan ELIZA test + antibody testing         Adrien Szekely

Beta-glucan testing                                               Michael Palmer     

Laboratory Session IV: Set-up and reading of antifungal susceptibility tests:

CLSI methodology                                                Zoë Brown

Etest for yeast and mould                                     Adrien Szekely

Dimorphic pathogens and other hazard group 3 fungi  Elizabeth Johnston

Laboratory Session V: Handling fungi at Containment Level (CL) 3, dimorphic
serology and National Collection of Pathogenic Fungi (NCPF):

 CL 3 Introduction                                                   Michael Palmer

 Dimorphic serology                                                Adrien Szekely

 NCPF                                                                    Elizabeth Johnston