Dr Guy Bewick

Dr Guy Bewick
Dr Guy Bewick
Dr Guy Bewick

FPhysiol

Senior Lecturer

Accepting PhDs

About
Email Address
g.s.bewick@abdn.ac.uk
Telephone Number
+44 (0)1224 437398
Office Address

Rm 6:18

Institute of Medical Sciences

School of Medicine, Medical Sciences & Nutrition

Foresterhill

Aberdeen AB25 2ZD

Scotland

United Kingdom

School/Department
School of Medicine, Medical Sciences and Nutrition

Biography

I graduated with a BSc in Zoology and Animal Physiology from the University of East Anglia in 1979. After 3 years working for the Ministry of Agriculture, Fisheries and Food inspecting export grain for insect infestation, I began my research career with a PhD position in the Department of Physiology, King’s College London with Dr David A Tonge. This initiated my research interest studying how appropriate nerve-muscle connections are made and then adapted to their diverse functions. I gained my PhD in 1986, investigating the regeneration of nerve-muscle connections. Subsequent postdoctoral work investigated other aspects of neuromuscular function. First, with Prof Glen Cottrell in St Andrews, investigating how simple (acetylcholine) and complex (neuropeptides) molecules, called 'neurotransmitters', are used simultaneously for neuromuscular signalling. Later, I worked in the Department of Physiology, University of Bristol with Dr Tony Ridge, studying the developmental pruning of nerve-muscle connections. SimBew spindle.jpgThis led to a major 2-year period at the University of Colorado Health Sciences Center, Denver, Colorado, USA. Here, with Dr Bill Betz, we developed technique for using FM1-43 and related dyes for fluorescent studies in living nerve terminals of the kinetics and distribution of the tiny neurotransmitter packets (vesicles) during activity. Between 1991 and 1994, I worked with Prof Clarke Slater in the Muscular Dystrophy Group Research Laboratories, Newcastle General Hospital investigating the role of muscular dystrophy-related structural proteins in building nerve-muscle specialisations. I joined the University of Aberdeen in 1994. While motor control of muscle is still a major interest, a collaboration with Dr Bob Banks, Durham University, is characterising how muscle stretch detectors (spindles) work. We discovered a powerful system for regulating their sensitivity. These studies will help our understanding of muscular dystrophy, MND/ALS, ataxia and hypertension.

Qualifications

  • FPhysiol Physiology 
    2020 - The Physiological Society 
  • PhD Neuroscience 
    1986 - King's College London 
  • BSc (Hons) Zoology & Animal Physiology 
    1979 - University of East Anglia 

Memberships and Affiliations

Internal Memberships

Lead Safety Co-ordinator, Institute of Medical Sciences, School of Medicine, Medical Sciences & Nutrition

Course Co-ordinator (Joint) SM3002 Frontiers of Biomedical Sciences

Personal Tutor

Aberdeen Cardiovascular and  Diabetes Centre

Translational Neuroscience Programme

External Memberships

Fellow of The Physiological Society of Great Britain and Ireland

Society for Neuroscience

British Neuroscience Association

Federation of European Neuroscience

British Society for Cell Biology

British Society for Developmental Biology

Scottish Neuroscience Group

Euan MacDonald Centre for MND Research

Latest Publications

View My Publications

Prizes and Awards

2016-2020 - Trustee, The Physiological Society

Research

Research Overview

We study how nerve signal strength is kept in the 'goldilocks zone' - not too strong, not too weak, but just right. In whatever we do, signalling adapts. This happens in both motor and sensory nerve endings. We study the nerve-muscle system, due to its importance for movement to us as animals, plus it is accessible, has large nerve endings and it changes use throughout life.

Understanding Movement Detection (Sensory Feedback)

Muscle length detector nerve terminals, labeled with
FM1-43 - a transmitter vesicle label.
(Dr Anna Simon, University of Aberdeen)

Here, our major focus is to find out why the nerve endings that detect movements of our limbs and blood vessels also secrete chemicals that act back to regulate their own sensitivity. With Dr Robert Banks (Durham University), we discovered this system of chemical secretion from synaptic-like vesicles. It seems important, as we showed that all such endings have this system. We are now trying to incorporate this into an integrated model of how these endings work, and so asking why they need such a control mechanism that can double the ending's sensitivity or even turn it off entirely!

 

Understanding Motor Nerve Function (Motor systems)

Transmitter vesicles (green) in motor nerve
terminal precisely overlie receptors on 
muscle fibre surface (red). 3D render.
(Dr Brian Reid, University of Aberdeen)

To move our muscles, an electrical signal triggers release of chemicals (neurotransmitter) from motor nerve endings on each muscle cell. These contacts form very early in development, yet the same nerve ending is present throughout life. We want to understand how the same nerve terminal knows how and when to change neurotransmitter release throughout life. It releases minute amounts in tiny baby muscles then enormous amounts in big adult muscles. Even further, they adapt to release much more in giant powerlifter muscle cells, or to maintain a constant release in muscles of distance runners. In all cases, neurotransmitter release adapts to be just enough to trigger muscle contraction in the size of the muscle cell it innervates, no matter how big or how often it is used. We want to understand the nerve-muscle signalling that adapts to meet these demands of growth, development and changes in in vivo activity.

 

Neurological disease treatments

iPSC-MN progenitors.jpg
Human ALS - stem cell-derived motor
nerve cells in culture.
(Abigail Dos Santos, PhD student)

These studies not only help us to understand how our wonderful nervous system works, but these natural regulatory systems are potential drug targets for treating symptoms in many diseases. Muscle weakness is often from poor neurotransmitter release or muscle response to it, e.g. myasthenia gravis, and congenital myasthenic syndromes. In motor neurone disease (aka MND or ALS), we think function in sick nerve terminals might be supported to extend the time  signalling is useful, alleviating symptoms for at least a little longer. Conversely, disrupted motor control (ataxia) seems to reflects poor sensory terminal function. Similar types of endings around blood vessels stretch and relax, monitoring our blood pressure. We are exploring their potential as drug targets for high blood pressure, which is currently the world's biggest mortality risk factor.

Research Areas

Accepting PhDs

I am currently accepting PhDs in Biomedical Sciences.


Please get in touch if you would like to discuss your research ideas further.

Email Me

Biomedical Sciences

Supervising
Accepting PhDs

Research Specialisms

  • Neuroscience
  • Physiology

Our research specialisms are based on the Higher Education Classification of Subjects (HECoS) which is HESA open data, published under the Creative Commons Attribution 4.0 International licence.

Current Research

Our two major lines of investigation are:

How do movement-detecting nerve endings work and adapt?

Our latest model of how these nerve endings work is this:

At rest, a chemical (glutamate) constantly dribbles from vesicles, keeping a receptor activated linked to PLD. This keeps the ending sensitive to movement. If blocked, the ending loses the ability to respond. Movement opens pores (channels) in the nerve terminal, letting positive sodium (Na+) ions in, making the inside positive. This opens different (voltage-sensitive) Na+ channels, producing a burst (afferent discharge) of electrical signals (action potentials) carried back to the central nervous system. These then open channels to allow calcium (Ca2+) in, then potassium (K+) out. K+ leaving moderates the afferent discharge frequency. Thus, the brain gets an urgent high-frequency signal when movement starts (meaning 'pay attention'), that slows to a modest level (meaning 'this is the movement happening'). Movement also lets Ca2+ into the terminal. This increases glutamate release, to maintain the terminal's ability to detect movement. We are investigating exactly how this glutamate system that we discovered regulates movement sensitivity. (Animation produced by Media Services and Medical Illustration, University of Aberdeen.© (2022) Guy Bewick, University of Aberdeen).

3968-7 spindle0002.jpg
3 length detectors in a skeletal muscle.
(Gillian Milne, Microscopy Facility,
University of Aberdeen) 

Mechanosensory endings tell us where our arms and legs are, whether we're touching anything, and monitor our blood pressure. Tiny vesicles, just like those releasing neurotransmitter, occur in all such endings throughout the animal kingdom. Their ubiquity suggests they are probably very important and we have shown that indeed they are. In muscle stretch receptors, we found they powerfully control ending sensitivity; from turning the ending off completely to doubling normal output! We now want to understand why this powerful regulation is needed. Then, we can understand if there are diseases associated with the system that were previously unsuspected, or might benefit from targeting with new drugs. For example, we are now testing if they can help us understand loss of co-ordination in diabetes, or mitochondrial diseases. We are even looking at this same system in stretch-sensitive endings around blood vessels. Might they be a new target for treating the most prevalent cardiovascular disease risk - high blood pressure, also known as hypertension.

How do motor nerve endings work and adapt?

Motor nerve terminal (yellow/green)
and recordings showing TGFbeta2
boosts transmitter release
nerve-muscle signalling.

We are studying how motor nerve terminals know what to do to activate their target. When we know that, these mechanisms may be targeted to treat diseases such as motor neurone disease (MND/ALS), or congenital muscle weakness. We have shown that motor nerve terminals on muscle cells 'learn' (i.e. become trained) to change neurotransmitter output, to suit their patterns of use. We want to know how this is done. How does a nerve terminal know how much transmitter to release to get the muscle to respond, even if the muscle cell changes size and how often it is used? How does it know how much neurotransmitter to store, depending on whether it is used often (e.g. marathon runners) or rarely (powerlifters). By understanding these mechanisms, they can become targets to treat motor nerve diseases, such as myasthenia (congenital myasthenia, myasthenia gravis) or sick motor neurones in motor neurone disease (MND/ALS). We found that a protein  (TGF-beta2) is secreted by the muscles during activity, causing the nerve terminal to release more transmitter, making it more effective. This is probably one signal between the two cell types to keep signalling 'just right' (not too much, not too little) in healthy muscles as they adapt to changing demands, such as growth, training and ageing. Now we are testing if this system can be targeted by drugs to increase signalling in conditions where nerve-muscle signalling is weakened by disease, such as early stage motor neurone disease, and myasthenias. An important new development is designing special cell culture dishes allowing human cells to be kept in just the right conditions, and to mimic human diseases in culture.

Past Research

1979-1986 (with Dr David Tonge, King's College London, UK).

Reinnervation of mammalian muscle after nerve damage nerves is effective (does occur, and does restore muscle contraction) but is not selective (will innervate the wrong muscle equally well). Conversely, in tailed amphibians, reinnervation by damaged motor nerves is both effective (produces muscle contraction) and specific (innervates only the correct muscle). This likely reflects the axolotl growing continuously, producing new nerves and muscles, that need to recognise each other throughout life.

1988-90 (with Prof Bill Betz, Univ of Colorado, USA).

We developed the technique for using FM1-43 and other styryl pyridinium dyes for visualising how living nerve terminals work during activity. This technique can be combined with a range of other recording techniques, too. It has >1000 citations, and has been adopted by laboratories all over the world. In addition to neuroscience, it has also been adapted for studies of surface vesicle trafficking in yeast, bacteria, plants, endocrine cells, and epithelial cells. We also determined the synaptic vesicles recycle time under continuous activity to be ~100 seconds.

1990-1994 (with Prof Clarke Slater, Univ of Newcastle upon Tyne, UK).

We showed the distribution of muscle proteins correlated with specific parts of the neuromuscular junction. Utrophin is associated with the crests of the postsynaptic folds, along with the acetylcholine receptors, while spectrin and dystrophin are in the depths of the postsynaptic folds, along with the voltage-gated Na channels.

Since arriving in Aberdeen

Motor nerve function:

We found that release from motor nerve terminals is adapted to their patterns use by adjusting the ratio of the number of transmitter quanta (i.e. vesicles = quantal content) released per stimulus, and the size of the vesicle pool (number of vesicles in the terminal). The recycle time was fixed under continuous activity at ~100 seconds. Thus, to maintain release, a terminal needs enough vesicles to last 100 seconds. With Dr Brian Reid (Aberdeen, UK, now UC Davis, USA), Profs Arild Nja, Terje Lomo (Oslo University, Norway) and Clarke Slater (Newcastle upon Tyne, UK).We then found that in the depletion of a neurotrophic factor NT-3, or blockade of the enzyme myosin light chain kinase (MLCK) vesicle trafficking is severely reduced, but transmitter release is much less affected. With Prof Phil Sheard & Marilyn Duxson, Univ of Otago, NZ).

Most recently, we showed that a peptide factor is secreted by the muscle cell, directly under the active motor nerve terminal, called transforming growth factor beta-2 (TGFb2), increases the efficiency of nerve-muscle communication. With Dr Sitt Wai Fong (Aberdeen, UK now Taipei University, Taiwan) and Prof Ian McLennan (Univ of Otago, NZ).

Lab-on-a-Chip (LOC) system to study ALS

C2C12s.jpg
Muscle cells awaiting contacts from
stem-cell derived human motor nerve
cells in LOC model of ALS.
(Claire Hetherington, PhD student).
iPSC-MN progenitors in chip.jpg
Stem cell-derived motor nerve cells
from ALS patient growing through
cross channels (right) to contact
muscle cells in adjacent chamber.
(Abigail Dos Santos, PhD student)

With Dr Claudiu Giuraniuc, we are now developing a Lab-on-a-Chip (LOC) system to study human motor neurone disease in a dish. If successful, we aim to develop a system  for rapid assay of drugs to promote survival of motor nerve cells and their muscle contacts in this devastating disease. As each set of stem cells comes from an individual with ALS, this system could also be the basis for personalised treatments for difference types of ALS, and even individual patients. At least, we hope so....

Supervision

My current supervision areas are: Biomedical Sciences.

We have one exciting PhD opportunity currently available (self-funding applicants only):

Mitochondria & motor control : nerves detecting muscle length are full of mitochondria. Is this why loss of co-ordination is so common in mitochondrial disease? This project explores mitochondria in terminal function and as potential therapeutic targets.

Publications

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  • Spatial relationships of utrophin, dystrophin, beta-dystroglycan and beta-spectrin to acetylcholine receptor clusters during postnatal maturation of the rat neuromuscular junction

    Bewick, G. S., Young, C., Slater, C. R.
    Journal of Neurocytology, vol. 25, no. 7, pp. 367-379
    Contributions to Journals: Articles
  • Illumination partly reverses the postsynaptic blockade of the frog neuromuscular junction by the styryl pyridinium dye RH414

    Bewick, G. S., Betz, W. J.
    Proceedings of the Royal Society of London. B, Biological Sciences, vol. 258, no. 1352, pp. 201-207
    Contributions to Journals: Articles
  • Restoration of motor unit properties and fiber type distribution in reinnervated axolotl skeletal muscle

    Bewick, G. S., Zammit, P. S., Tonge, D. A.
    Developmental Biology, vol. 158, no. 2, pp. 288-300
    Contributions to Journals: Articles
  • Comparison of FM1-43 staining patterns and electrophysiological measures of transmitter release at the frog neuromuscular junction

    Betz, W. J., Ridge, R. M., Bewick, G. S.
    Journal of Physiology - Paris, vol. 87, no. 3, pp. 193-202
    Contributions to Journals: Articles
  • Optical monitoring of transmitter release and synaptic vesicle recycling at the frog neuromuscular junction

    Betz, W. J., Bewick, G. S.
    The Journal of Physiology, vol. 460, pp. 287-309
    Contributions to Journals: Articles
  • Relationship of a dystrophin-associated glycoprotein to junctional acetylcholine receptor clusters in rat skeletal muscle

    Bewick, G. S., Nicholson, L. V., Young, C., Slater, C. R.
    Neuromuscular Disorders, vol. 3, no. 5-6, pp. 503-506
    Contributions to Journals: Articles
  • Intracellular movements of fluorescently labeled synaptic vesicles in frog motor nerve terminals during nerve stimulation

    Betz, W. J., Bewick, G. S., Ridge, R. M.
    Neuron, vol. 9, no. 5, pp. 805-813
    Contributions to Journals: Articles
  • Different distributions of dystrophin and related proteins at nerve-muscle junctions

    Bewick, G. S., Nicholson, L. V., Young, C., O'Donnell, E., Slater, C. R.
    Neuroreport, vol. 3, no. 10, pp. 857-860
    Contributions to Journals: Articles
  • Activity-dependent fluorescent staining and destaining of living vertebrate motor nerve terminals

    Betz, W. J., Mao, F., Bewick, G. S.
    Journal of Neuroscience, vol. 12, no. 2, pp. 363-375
    Contributions to Journals: Articles
  • Optical analysis of synaptic vesicle recycling at the frog neuromuscular junction

    Betz, W. J., Bewick, G. S.
    Science, vol. 255, no. 5041, pp. 200-203
    Contributions to Journals: Articles
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