RNA-Seq for de novo Transcriptome Assembly

Comprehensive service typically 8-26 weeks:

  1. QC of data.
  2. OPTIONAL: Removal of contamination reads e.g. from host.
  3. Assembly:
    1. Assembly with different assembler tools.
    2. Assembly metrics and comparisons. Selection of best assembly.
  4. Annotation of assembly:
    1. Annotation with Trinotate.
    2. Comparison to closely related species. 
  5. OPTIONAL: Iterative biology drive, in depth annotation of specific loci of interest.
  6. Publication quality visualisation and reporting.
  7. Input to publication, including revisions and resubmissions.