BIS-Seq: Methylation Calling

Comprehensive service typically 4-20 weeks:

  1. QC of data.
  2. Alignment to reference, methylation calling of bisulphite-treated samples and generation of raw methylation results/standard reports per strand and methylation context:
    1. OPTIONAL: Consolidation of raw methylation results into user-defined reports for subsequent downstream analysis.
    2. OPTIONAL: Alignment to reference (good quality reference), variant calling and filtering of non-bisulphite treated samples. 
  3. Publication quality visualisation and reporting.
  4. Input to publication, including revisions and resubmissions.

BIS-RNA-Seq

Comprehensive service typically 10-24 weeks:

  1. QC of data.
  2. Alignment to reference, methylation calling of bisulphite-treated samples and generation of raw methylation results/standard reports per strand and methylation context (2 weeks):
    1. OPTIONAL: Consolidation of raw methylation results into user-definied reports for subsequent downstream analysis.
    2. OPTIONAL: Alignment to reference (good quality reference), variant calling and filtering of non-bisulphite-treated samples.
  3. Differential methylation analysis and reporting:
    1. OPTIONAL: Annotation of most significantly differentially methylated transcripts.
  4. Publication quality visualisation and reporting.
  5. Input to publication, including revisions and resubmissions.