Liquid Chromatography Mass Spectrometry (LCMS)
Liquid Chromatography Mass Spectrometry
The Institute uses electrospray ionisation mass spectrometry to support research into programmes aimed at understanding the balance between diet and human health. The LC/MS/MS instruments are used for the quantification of a range of metabolites by Multiple Reaction Monitoring (MRM), for the identification of proteins and de novo peptide sequencing following 2D PAGE electrophoresis to support proteomic research. The section has 4 instruments; an AB Sciex Q-Trap 2000, an AB Sciex Q-Trap 4000, an AB Sciex API 3200 and an Agilent 6490.
Facilities support research in:
- Analysis of small molecules (phenolics, flavanoids, phospholipids)
- Protein identification by peptide sequencing
- Metabolite identification and quantification
Latest Publications
Gratz, S.W., Duncan, G., Richardson, A.J. Applied and Environmental Microbiology 2013 Vol. 79 pp 1821-1825. The Human Fecal Microbiota Metabolizes Deoxynivalenol and Deoxynivalenol-3-Glucosidec and May Be Responsible for Urinary Deepoxy-Deoxynivalenol.
Maloney, C.A., Hay, S.M., Reid, M.D., Duncan, G.J., Nicol, F., Sinclair, K.D., Rees, W.D.Genes and Nutrition 2013 Vol. 8 pp181-190.A Methyl-deficient Diet Fed to Rats During the Pre- and Peri-conception Periods of Development Modifies the Hepatic Proteome in the Adult Offspring.
Russell, W.R., Duncan, S.H., Scobbie, L., Duncan, G., Cantlay, L., Calder, A.G., Anderson, S.E., Flint, H.J.Molecular Nutrition and Food Research 2013 Vol. 57 pp 523-535. Major Phenylpropanoid-derived Metabolites in the Human Gut Can Arise From Microbial Fermentation of Protein.
Kaniewska, J., Bestwick, C.S., Russell, W.R., Scobbie, L., Duncan, G., Milne, L., Duthie, S.J. Colorectal cancer: do phytophenols in the human diet prevent genomic instability?, Mutagenesis, 27 (6) November 2012 pp 802-803.
Rodrguez-Gutirrez, G., Duthie, G. G., Wood, S., Morrice, P., Nicol, F., Reid, M., Cantlay, L. L., Kelder, T., Horgan, G. W., Fernndez-Bolaos Guzmn, J. Alperujo extract, hydroxytyrosol, and 3,4-dihydroxyphenylglycol are bioavailable and have antioxidant properties in vitamin E-deficient rats-a proteomics and network analysis approach, Molecular Nutrition and Food Research, 56 (7) July 2012 pp. 1137-1147
Robins, S.P., Wilson, N.J., Duncan, G. Anal. Biochem. 2012 in press. Determination of Immature and Mature Collagen Cross-links and the Desmosines of Elastin by Liquid Chromatography-Tandem Mass Spectrometry in Tissues and Urine.
Rodriguez-Gutierrez, G., Duthie, G.G., Wood, S., Morrice, P.C., Nicol, F., Reid, M.D., Cantlay, L.L., Kelder, T., Horgan, G.W., Fernandez-Bolanos Guzman, J., De Roos, B. Mol. Nut. & Food Res. 2012 in press. Alperujo Extract, Hydroxytyrosol and 3,4-Dihydroxyphenylglycol are Bioavailable and have Antioxidant Properties in Vitamin-E Deficient Rats – A Proteomic and Network Analysis Approach.
Rungapamestry, V., McMonagle, J., Reynolds, C., Rucklidge, G.J., Reid, M.D., Duncan, G.J., Ross, K., Horgan, G.W., Toomey, S., Moloney, A.P., De Roos, B., Roche, H. Proteomics 2012 Vol. 12 pp 461-476. Inter-organ Proteomic Analysis Reveals Insights into the Molecular Mechanisms Underlying the Anti-diabetic Effects of cis-9, trans-11 Conjugated Linoleic Acid in ob/ob Mice.
Russell, W.R., Gratz, S.W., Duncan, S.H., Holtrop, G., Ince, J., Scobbie, L., Duncan, G., Johnstone, A.M., Lobley, G.E., Wallace, R.J., Duthie, G.G., Flint, H.J. Am. J. Clin. Nut. 2011 Vol 93 pp 1062-1072. High-Protein, Reduced-Carbohydrate Weight-Loss Diets to Promote Metabolite Profiles Likely to be Detrimental to Colonic Health.
De Roos, B., Zhang, X., Gutierrez, G.R., Wood, S., Rucklidge, G.J., Reid, M.D., Duncan, G.J., Cantlay, L.L., Duthie, G.G., O’Kennedy, N. Eur. J. Nut. 2011 Vol. 50 pp 553-562. Anti-platelet Effects of Olive Oil Extract: In Vitro Functional and Proteomic Studies.
Padidar, S., Farquharson, A.J., Williams, L.M., Hoggard, N., Reid, M.D., Duncan, G.J., Drew, J.E. Dig. Dis. Sci. 2011 Vol. 56 pp 1028-1036. Impact of Obesity and Leptin on Protein Expression Profiles in Mouse Colon.