BA (University of Cambridge, 1989), PhD (MRC Laboratory of Molecular Biology, Cambridge, 1994)
BBSRC-funded PhD studentship available! If you are interested in joining the lab as a PhD student please contact Anne Donaldson, sending your CV and explaining your interest in this specific research area. Deadline 14 Dec 2015.
Anne Donaldson studied Natural Sciences at the University of Cambridge, completed her PhD at the MRC Laboratory of Molecular Biology, then moved as a NATO/SERC postdoctoral fellow to the University of Washington in Seattle. Anne established her lab as a Royal Society University Research Fellow at the University of Dundee, and in 2001 Anne was chosen as an EMBO Young Investigator. The Donaldson laboratory has been at the University of Aberdeen Institute of Medical Sciences since 2003. The lab is funded by Cancer Research UK and the BBSRC.
Anne teaches on several Aberdeen University Molecular & Cell Biology courses, and from 2006-13 was Examinations Officer & Chair of Examinations Boards (Molecular & Cell Biology). Anne serves as Honorary Treasurer of the Genetics Society, as Cellular & Molecular Biology Subject Editor for Royal Society Open Science, and as an Organizer of the Cold Spring Harbor Eukaryotic DNA Replication & Genome Maintenance meeting.
Human cells contain 1.8 metres of DNA in a nucleus only about 6 microns in diameter. During chromosome replication this entire length of DNA must be duplicated exactly once with perfect accuracy, so that the strands can be disentangled and precisely segregated to the daughter cells. The DNA is extremely vulnerable to damage during this process, and cells must deal with thousands of potentially lethal DNA damage events every single day. Members of the Donaldson lab investigate the controls over DNA replication and damage repair. Understanding chromosome maintenance will suggest new therapeutic strategies in the fight against cancer, as well as illuminating the basic mechanisms at the heart of the cell division cycle.
The budding yeast S. cerevisiae provides an excellent model organism for studying the fundamentals of chromosome biology, because of the remarkable molecular genetics tools available for this system. DNA replication initiates at multiple sites on each chromosome called replication origins. We used comparative genomics and molecular genetics to identify yeast replication origin sequences genome-wide, allowing us to create an interactive database of yeast replication origins, called OriDB (www.oridb.org).
Our current focus of interest is understanding the molecular machinery controlling origin initiation, replication fork progression, and chromosome maintenance. We use a combination of advanced proteomic, genomic and microscopy methods to investigate the cellular components that regulate these DNA replication and repair processes. It becomes increasingly clear that the molecular machinery of chromosome maintenance is conserved from yeast to human. We are extending our investigations to test how the mechanisms we discover in yeast also operate in mammalian cells to ensure proper genome maintenance.
Kate Clark - postdoc
Vamsi Krishna Gali - postdoc
Javier Garzon -postdoc
Shin-ichiro Hiraga - senior research fellow
Sylwia Kedziora - PhD student
Lotte Watts - PhD student
Julie Brookes - technician
FORMER LAB MEMBERS
Andrew Cosgrove - PhD student 2001-05
Sotirios Botsios - PhD student 2007-2010
Hani Ebrahimi - PhD student 2005-09
Takashi Kubota - postdoc 2008-2014/MRC Career Development Fellow 2014-19
Huiyong Lian - postdoc 2008-2011
Amanda Mackenzie - Masters student 2009-10
Conrad Nieduszynski - postdoc/Leverhulme Research Fellow 2000-07
Douglas Robertson - PhD student 2003-07
Akila Sridhar - PhD student 2009-2014